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Bis-Tris Methane

Bis-Tris Methane is generally used as a buffering agent in biochemistry. Bis-Tris Methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25°C. It is an effective buffer between the pH 5.8 and 7.3.

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Diethanolamine is an organic compound. This colorless liquid is polyfunctional, being a secondary amine and a diol. Like other organic amines, diethanolamine acts as a weak base. It is used as a surfactant and a corrosion inhibitor. It is used to remove hydrogen sulfide and carbon dioxide from natural gas. Diethanolamides are common ingredients in cosmetics and shampoos added to confer a creamy texture and foaming action.

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Formamide is an amide derived from formic acid. It is a clear liquid which is miscible with water and has an ammonia-like odor. It is chemical feedstock for the manufacture of sulfa drugs, other pharmaceuticals, herbicides, pesticides and the manufacture of hydrocyanic acid. It has been used as a softener for paper and fiber. It is a solvent for many ionic compounds. It has also been used as a solvent for resins and plasticizers.Formamide is also a constituent of cryoprotectant vitrification mixtures used for cryopreservation of tissues and organs.Formamide is also used as an RNA stabiliser in gel electrophoresis by deionizing RNA. In capillary electrophoresis, it is used for stabilizing (single) strands of denatured DNA.Formamide, in its pure state, has been used as an alternative solvent for the electrostatic self-assembly of polymer nanofilms. It is also used for prehybridization and hybridization of biotinylated nucleic acid probes to nucleic acids fixed on membranes.

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N-(2-Acetamido)-2-Aminoethanesulfonic Acid

N-(2-Acetamido)-2-Aminoethanesulfonic Acid is one of Good's buffers developed in the 1960s to provide buffers with pH ranging from 6.15-8.35 for use in various applications. With a pKa of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. It had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis.[2] ACES use in isoelectric focusing of proteins has also been documented.

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Tris(hydroxymethyl)aminomethane is extensively used in biochemistry and molecular biology as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines. It is also used as a primary standard to standardize acid solutions for chemical analysis.

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