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5-Nitro-o-toluidine is used in organic synthesis.

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5-Nitroacenaphthene is intermediates of high dye acid Yellow FF and disperse Yellow which is also used as a photoresist sensitizer.

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5-Nitroindole is a hydrophobic aromatic compound, and can be used as a universal base analog in oligonucleotides.It does not discriminate between the four natural nucleotide bases in duplex formation,but does contribute to duplex stability through base-stacking interactions.5-Nitroindole directs random incorporation of any base when used as a template for DNA polymerase and partially blocks enzyme processivity.

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5-Nitroisatin is used as an intermediate for agrochemicals or medicines.

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