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1-Anilino-8-Naphthalenesulfonate is a fluorescent dye with high affinity towards hydrophobic surfaces of proteins. It is useful for determining the affinity of hydrophobic ligands to their corresponding binding proteins. It undergoes a blue shift and fluorescence increases when it binds to non-polar regions of a protein surface or membranes. 1-Anilino-8-Naphthalenesulfonate used as a probe to monitor protein-folding.

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