Agarose Properties, Molecular Formula, Applications - WorldOfChemicals

Agarose Properties

Agarose

2-Chloro-3-Picoline-5-Boronic Acid-Product_Structure
Molecule Structure Image

Description

 

Agarose is a linear polymer with a molecular weight of about 120,000, consisting of alternating D-galactose and 3,6-anhydro-L-galactopyranose linked by ?-(1?3) and ?-(1?4) glycosidic bonds. An agarose is a polysaccharide polymer material, generally extracted from seaweed. Agarose is one of the two principal components of agar, and is purified from agar by removing agar's other component, agaropectin. Agarose is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Agarose exhibits the phenomenon of thermal hysteresis in the liquid-to-gel transition. Agarose is a preferred matrix for work with proteins and nucleic acids as it has a broad range of physical, chemical and thermal stability, and its lower degree of chemical complexity also makes it less likely to interact with biomolecules. Agarose is most commonly used as the medium for analytical scale electrophoretic separation in agarose gel electrophoresis. Agarose gel electrophoresis is the routine method for resolving DNA in the laboratory. It can also be used to separate large protein, and it is the preferred matrix for the gel electrophoresis of particles with effective radii larger than 5-10 nm. Agarose gels are cast horizontally in a mold, and when set, usually run horizontally in a submarine mode in buffer. Agarose gel matrix is often used for protein purification, for example, in column-based preparative scale separation as in gel filtration chromatography, affinity chromatography and ion exchange chromatography.

Chemical Properties

Appearance White crystalline powder
CAS Number 9012-36-6
HS Code 130239000
Lab Grade LR
Molar Mass 225.24 g/mol
Molecular Formula C10H15N3O3
Synonyms Qa-Agarose(Tm);Serva Agarose;Sepharose(R) 2B;Sepharose(R) 4B
www.worldofchemicals.com uses cookies to ensure that we give you the best experience on our website. By using this site, you agree to our Privacy Policy and our Terms of Use. X