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Auramine O is a diarylmethane dye used as a fluorescent stain. It can be used to stain acid-fast bacteria, where it binds to the mycolic acid in its cell wall, in a way similar to Ziehl-Neelsen stain. It can also be used as a fluorescent version of Schiff reagent. Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis. It can be also used as an antiseptic agent.
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