ELISA Substrates' new optimization methods to drive horseradish peroxidase market

11:04 AM, 30th January 2021
Researchers are seeking the help of companies in the horseradish peroxidase market to develop treatments and vaccines for COVID-19 (coronavirus). (Representative Image © Pixabay GmbH)

Researchers and scientists are seeking the help of companies in the horseradish peroxidase market to develop treatments and vaccines for COVID-19 (coronavirus). Companies are increasing efforts to provide remote support for SARS-CoV-2 research. They are appointing a team of experts that help in research activities. As such, there is an urgent need to accelerate vaccine development to fight COVID-19. Additional support with the help of instrument supply is helping to scale the development of vaccines.

Leading companies in the horseradish peroxidase market are gaining an edge over emerging players by providing enzymes and other products & services to end users in areas where travel is restricted. On the other hand, webinars are playing a crucial role in educating researchers and biologists about various steps required for advanced vaccine development. As such, researchers are gaining insights in understanding the biology of SARS-CoV-2 in order to achieve next-gen sequencing in samples.

Enzyme Immobilization Technique Helps Optimize Specific Properties

Horseradish peroxidase is being pervasively used as a biosensor and is being used in clinical chemistry, owing to its advantages of high substrate specificity and catalytic activity. As such, horseradish peroxidase and other enzymes such as glucose oxidase and cholesterol oxidase are increasingly replacing conventional chemical catalysts, since the latter suffer from shortcomings of high cost and low stability. This bodes well for the growth of the horseradish peroxidase market, which is projected to advance at a healthy CAGR of ~7% during the forecast period.

Companies in the horseradish peroxidase market are overcoming challenges pertaining to enhancement of catalytic activity and stability with the help of the enzyme immobilization technique. The enzyme immobilization technique is one of the most cost-efficient ways of enhancing specific properties in horseradish peroxidase. Moreover, this technique is being highly publicized to minimize the enzyme inhibition effects of horseradish peroxidase.

ELISA Substrate Selection Determines End Results in Assay Systems

Manufacturers in the horseradish peroxidase market are gaining proficiency to develop enzymes with a range of sensitivities that play an instrumental role in different detection methods. They are increasing awareness about careful selection of ELISA substrates in order to obtain best results in assay systems. As such, ELISA substrates differ in price, ease-of-use, and sensitivity. Hence, researchers must opt for a variety of procedures to bring ELISA substrates in the working condition. The preparation of substrates plays a key role in detecting signals in assay systems. However, companies in the horseradish peroxidase market are increasing efforts to boost the availability of substrates that are in ready-to-use forms.

There is a growing awareness about various optimization methods for ELISA substrates, which help to deliver better results in assay systems. Manufacturers are increasing their production capabilities to develop chromogenic substrates for ELISA, which are used in combination with horseradish peroxidase enzyme (HRP).

Strategic Acquisitions Help Leverage Opportunities in Sample Preparation Technologies

The horseradish peroxidase market is predicted to surpass the value of $100 mn by 2030. This is an important revelation, since companies are securing stakes of other companies even amidst the ongoing coronavirus pandemic. For instance, in March 2020, Thermo Fisher Scientific - a US provisioner of reagents, consumables, and scientific instrumentation, announced the acquisition of QIAGEN— a provider of sample and assay technologies for molecular diagnostics to explore opportunities in sample preparation technologies.

In order to achieve increased sensitivity, the technique of chemifluorescence is being used for ELISA reactions. Thus, companies in the horseradish peroxidase market are becoming increasingly aware about this technique and has helped to increase the magnitude of sensitivity in ELISA reactions. They are using photomultiplier tubes with fluorescence detection in order to achieve stability in reactions.

Recombinant Protein Production Accelerates Enzyme Preparation

Apart from blotting and assay systems, horseradish peroxidase is being used for wastewater treatment and as a reagent in organic synthesis. Since horseradish peroxidase is extracted from armoracia rusticana roots, seasonal availability and long cultivation times pose as hurdles for manufacturers. Hence, manufacturers in the horseradish peroxidase market should aim for uniform enzyme preparation with the help of saccharomyces cerevisiae or pichia pastoris, as host organisms for recombinant protein production since yeasts are easy to cultivate.

Horseradish peroxidase holds promising potentials in therapeutic use, owing to its stability at specific temperatures. As such, companies in the horseradish peroxidase market are increasing their focus in medical applications. They are increasing their R&D activities to innovate in non-glycosylated recombinant horseradish peroxidase with the help of site-directed mutagenesis of amino acids.

ABTS Reagents Mimic Properties of TMB in Prolonged Reaction Time Durations

ABTS is being highly publicized as a safe and sensitive substrate for horseradish peroxidase-based ELISA systems. Hence, companies in the horseradish peroxidase market are increasing the availability of ABTS reagents. For instance, Merck - a US multinational pharmaceutical company has a wide range of product portfolio in blue green color ABTS single reagents that enhance enzymatic activities. Improved versions of ABTS mimic the properties of TMB if a reaction is allowed to progress for prolonged periods.

Companies in the horseradish peroxidase market are increasing their production capabilities to develop room-temperature stable single component ABTS reagents that are being pervasively used in ELISA procedures. Thus, researchers are opting for non-organic formulations for horseradish peroxidase detection systems. Manufacturers are developing ABTS reagents that are largely optimized for ELISA assays in microwell plates or test tubes.

Key Drivers of Horseradish Peroxidase Market

Research organizations across the public sector and pharmaceutical & biotech industries are investing in research & development of new drugs to address the increase in incidence of rare and chronic diseases. The biopharmaceutical industry in the US invested an estimated $102 bn in R&D in 2018.

In general, for medical diagnostics, it is important to have a stable and robust biopharmaceutical system employing enzymes without any variations in the glycosylation pattern to guarantee reliable and reproducible results. Hence, horseradish peroxidase has become a key component in biopharmaceutical research.

Additionally, horseradish peroxidase evolved as a biocatalyst in the research filed due to chemical properties. Hence, it is widely utilized in the study of different pharmaceuticals and other fine chemicals products.

Drawbacks of HRP as Enzyme in Chemiluminescence: Key Restraint

The first minor drawback of using horseradish peroxidase is that it could result in non-specific staining that may occur from the endogenous peroxidase activity observed in certain tissues or cells. When thin frozen section of tissues are made, these often reveal a mild to moderate amount of endogenous peroxidase activity. This is likely to act as a deterrent for researchers to use horseradish peroxidase.

Penetration into Novel Applications: Key Opportunities

A number of gene therapy strategies has been proposed for the treatment of cancer. Horseradish peroxidase utilizes hydrogen peroxide to oxidize both organic and inorganic compounds. Several studies have shown that the cytotoxicity was also observed under conditions of severe hypoxia (catalyst induced anoxia) indicating that the HRP/paracetamol combination could be suitable for hypoxia-targeted gene therapy. This, in turn, is expected to create an opportunity for suppliers of horseradish peroxidase.

Competition Landscape

This report profiles major players in the global horseradish peroxidase market based on various attributes such as company overview, financial overview, product portfolio, business strategies, and recent developments

The global horseradish peroxidase market is highly fragmented, owing to the presence of numerous international as well as regional players

Leading players operating in the global horseradish peroxidase market include

Abcam Plc, Thermo Fisher Scientific Inc, Bio-Rad Laboratories, Merck KGaA, BBI Solutions, Takara Bio Company, Promega Corporation, Faizyme Laboratories, F. Hoffmann-La Roche Ltd, TOYOBO Co Ltd, Oriental Yeast Co Ltd and GenScript.


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