Lighting up disease-carrying mosquitoes

Lighting up disease-carrying mosquitoes

9:18 AM, 28th March 2016
Lighting up disease-carrying mosquitoes
Red means a disease is present to Sandia National Laboratories’ researchers Cameron Ball and Robert Meagher as they test their QUASR, for quenching of unincorporated amplification signal reporters, technique to detect the presence of malaria and viruses like West Nile.

LIVERMORE, US: Mosquitoes are deadly efficient at spreading disease. Despite vaccines and efforts to eradicate the pesky insects, they continue to infect humans with feared diseases like Zika virus, malaria and West Nile virus.

Gaining the upper hand on mosquitoes requires speed. Their life cycle is typically two weeks or less and they need only warm weather and standing water to breed.

Robert Meagher, a chemical engineer at Sandia National Laboratories, has developed a simple technique for simultaneously detecting RNA from West Nile and chikungunya virus in samples from mosquitoes. He is now working to add the ability to screen for Zika virus.

“Our ultimate goal is to develop an autonomous device to passively monitor for mosquito-borne diseases,” Meagher explained. “But first you need an assay that is more robust than the gold standard in a laboratory and that has a very low false-positive rate.”

Brighter than the sun

Meagher and Sandia colleagues Yooli Light, Chung-Yan Koh and postdoctoral researcher Cameron Ball describe the technique in a paper published online in Analytical Chemistry, “Quenching of unincorporated amplification signal reporters (QUASR) in RT-LAMP enables bright, single-step, closed-tube and multiplexed detection of RNA viruses.”

They explored reverse-transcription loop mediated isothermal amplification (RT-LAMP), an isothermal nucleic acid amplification technique used instead of the more common polymerase chain reaction (PCR) for low-cost or point-of-care diagnostics for infectious diseases. However, in its usual form, RT-LAMP requires too many manipulations or elaborate instrumentation to be suitable for field use or an autonomous device.

RT-LAMP relies on primers, DNA fragment sequences, that bind to a target — the RNA of the virus of interest — and then generate a large amount of DNA. By fluorescently labeling the primer, the new DNA glows as it is produced. If the primer doesn’t find the viral RNA, there is no glow.

The Sandia researchers added a different DNA fragment sequence called a quench probe that complements a short stretch of primer. As the reaction takes place, the quench probe suppresses any unincorporated primer. This amplifies the positive signal up to 10 times brighter than a negative signal. Because the signal is so bright, QUASR can screen up to three different targets simultaneously, saving time and money.

“We didn’t expect a signal that bright, so we came up with the name QUASR, inspired by quasars, the extremely luminous celestial objects that can be a trillion times brighter than the sun,” Meagher said.

QUASR is simple enough to be used in a field laboratory. The Sandia team is working to incorporate QUASR into a handheld device.

QUASR enables rapid response

Vector control districts stand to benefit greatly from QUASR. Such districts are typically the part of public health agencies that monitors and controls disease-carrying insects locally.

Currently, detecting disease-carrying mosquitoes is a complex and lengthy process. A field worker in a vector control district collects and prepares mosquito samples, which are then sent to a lab for testing using sophisticated PCR techniques.

Using QUASR, either in a portable microfluidic device or simple field laboratory, a user can get a definitive yes or no result in about half an hour. More details like the amount of viral RNA present in the sample would still require laboratory PCR.

“This would allow those in the field to make quick decisions on mosquito abatement that can prevent the spread of disease,” Meagher said.

The work was funded by Sandia’s Laboratory Directed Research & Development program.

© Sandia National Laboratories News

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