New imaging technique paints tissue samples with light

New imaging technique paints tissue samples with light

7:41 AM, 25th March 2015
New imaging technique paints tissue samples with light
Breast tissue is computationally stained using data from infrared imaging without actually staining the tissue, enabling multiple stains on the same sample.

CHAMPAIGN, US: One infrared scan can give pathologists a window into the structures and molecules inside tissues and cells, enabling fast and broad diagnostic assessments, thanks to an imaging technique developed by University of Illinois researchers and clinical partners.

Using a combination of advanced microscope imaging and computer analysis, the new technique can give pathologists and researchers precise information without using chemical stains or dyes. The research is led by Rohit Bhargava, Professor, University of Illinois. “Any sample can be analyzed for desired stains without material cost, time or effort, while leaving precious tissue pristine for downstream analyses,” said Bhargava.

To study tissue samples, doctors and researchers use stains or dyes that stick to the particular structure or molecule they are looking for. Staining can be a long and exacting process, and the added chemicals can damage cells. Doctors also have to choose which things to test for, because it’s not always possible to obtain multiple samples for multiple stains from one biopsy.

The new, advanced infrared imaging technique uses no chemical stains, instead scanning the sample with infrared light to directly measure the chemical composition of the cells. The computer then translates spectral information from the microscope into chemical stain patterns, without the muss or fuss of applying dyes to the cells.

“We’re relying on the chemistry to generate the ground truth and act as the ‘supervisor’ for a supervised learning algorithm. One of the bottlenecks in automated pathology is the extensive processing that must be applied to stained images to correct for staining artifacts and inconsistencies. The ability to apply stains uniformly across multiple samples could make these initial image processing steps significantly easier and more robust,” said David Mayerich, first author of the study. Mayerich was a post-doctoral fellow at the Beckman Institute and now is a professor at the University of Houston.

The researchers reproduced a wide array of molecular stains by computationally isolating the spectra of specific molecules. This allows the user to simply tune to a required stain, for as many different stains as are necessary – all without damaging the original tissue sample, which can then be used for other tests.

“This approach promises to have immediate and long-term impact in changing pathology to a multiplexed molecular science – in both research and clinical practice,” said Bhargava.


© University of Illinois News



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