UC Berkeley scientists taken proteins from nerve cells could provide flexible, sensitive coating

Proteins from nerve cells could provide flexible, sensitive coating

11:39 AM, 15th October 2014
UC Berkeley scientists taken proteins from nerve cells could provide flexible, sensitive coating
The height of the new protein brush, made from neurofilament-derived proteins, can be precisely controlled with protein-digesting enzymes, or proteases. The protease thrombin cut the brush superficially at the red cross marks, resulting in a negligible change to the height of the brush.

CALIFORNIA, US: UC Berkeley scientists have taken proteins from nerve cells and used them to create a “smart” material that is extremely sensitive to its environment. This could give birth to a flexible, sensitive coating that is easy and cheap to manufacture in large quantities. The work could lead to new types of biological sensors, flow valves and controlled drug release systems, the researchers said. Biomedical applications include microfluidic devices that can handle and process very small volumes of liquid, such as samples of saliva or blood, for diagnostics.

“This work represents a unique convergence of the fields of biomimetic materials, biomolecular engineering and synthetic biology. We created a new class of smart, protein-based materials whose structural principles are inspired by networks found in living cells,” said Dr. Sanjay Kumar, Professor of Bioengineering, UC Berkeley.

Kumar’s research team set out to create a biological version of a synthetic coating used in everyday liquid products, such as paint and liquid cosmetics, to keep small particles from clumping together. The synthetic coatings are often called polymer brushes because of their bristle-like appearance when attached to the particle surface.

To create the biological equivalent of a polymer brush, the researchers turned to neurofilaments, pipe cleaner-shaped proteins found in nerve cells. By acting as tiny, cylindrical polymer brushes, neurofilaments collectively assemble into a structural network that helps keep one end of the nerve cell propped open so that it can conduct electrical signals.

“We co-opted this protein and turned it into a polymer brush by cloning a portion of a gene that encodes one of the neurofilament bristles, re-engineering it such that we could attach the resulting protein to surfaces in a precise and oriented way, and then expressing the gene in bacteria to produce the protein in large, pure quantities. We showed that our ‘protein brush’ had all the key properties of synthetic brushes, plus a number of advantages,” said Kumar.

Kumar noted that neurofilaments are good candidates for protein brushes because they are intrinsically disordered proteins, so named because they don’t have a fixed 3-D shape. The size and chemical sequence of these hair-like proteins are far easier to control when compared with their synthetic counterparts.

“In biology, precision is critical. Proteins are generally synthesized with the exact same sequence every time; the length and biochemical order of the protein sequence affects all of its properties, including structure and the ability to bind to other molecules and catalyze biochemical reactions. This kind of sequence precision is difficult if not impossible to achieve in the laboratory using the tools of chemical synthesis. By harnessing the precision of biology and letting the bacterial cell do all the work for us, we were able to control the exact length and sequence of the bristles of our protein brush,” said Kumar.

The researchers showed that the protein brushes could be grafted onto surfaces, and that they dramatically expand and collapse in reaction to changes in acidity and salinity. Materials that are environmentally sensitive in this way are often referred to as “smart” materials because of their ability to adaptively respond to specific stimuli.

 

© University of California, Berkeley News

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