Carnegie Mellon University Researchers create self-assembling protein, polymer nanostructures

Scientists create self-assembling protein, polymer nanostructures

9:53 AM, 4th August 2014
Carnegie Mellon University Researchers create self-assembling protein, polymer nanostructures
Carnegie Mellon chemists create nanofibers using unprecedented new method.

PITTSBURGH, US: Researchers from Carnegie Mellon University have developed a novel method for creating self-assembled protein/polymer nanostructures that are reminiscent of fibers found in living cells. The work offers a promising new way to fabricate materials for drug delivery and tissue engineering applications. The findings were published in Angewandte Chemie International.

“We have demonstrated that, by adding flexible linkers to protein molecules, we can form completely new types of aggregates. These aggregates can act as a structural material to which you can attach different payloads, such as drugs. In nature, this protein isn’t close to being a structural material,” said Tomasz Kowalewski, Professor, Carnegie Mellon’s College of Science.

The building blocks of the fibers are a few modified green fluorescent protein (GFP) molecules linked together using a process called click chemistry. An ordinary GFP molecule does not normally bind with other GFP molecules to form fibers. But when Carnegie Mellon graduate student Saadyah Averick, working under the guidance of Krzysztof Matyjaszewski, the JC Warner Professor of Natural Sciences and University Professor of Chemistry in CMU’s Mellon College of Science, modified the GFP molecules and attached PEO-dialkyne linkers to them, they noticed something strange - the GFP molecules appeared to self-assemble into long fibers. Importantly, the fibers disassembled after being exposed to sound waves, and then reassembled within a few days. Systems that exhibit this type of reversible fibrous self-assembly have been long sought by scientists for use in applications such as tissue engineering, drug delivery, nanoreactors and imaging.

“This was purely curiosity-driven and serendipity-driven work. But where controlled polymerization and organic chemistry meet biology, interesting things can happen,” said Kowalewski.

The research team observed the fibers using confocal light microscopy, confirmed their assembly using dynamic light scattering and studied their morphology using atomic force microscopy (AFM). They also observed that the fibers were fluorescent, indicating that the GFP molecules retained their 3-D structure while linked together.

To determine what processes were driving the self-assembly, Matyjaszewski and Kowalewski turned to Anna Balazs, Distinguished Professor of Chemical Engineering and the Robert v d Luft, Professor at the University of Pittsburgh. A leading expert in modeling the dynamics and mechanical properties of mesoscale systems, Balazs ran a computer simulation of the GFP molecules’ self-assembly process using a technique called dissipative particle dynamics, a type of coarse-grained molecular dynamics method. The simulation confirmed the modified GFP’s tendency to form fibers and revealed that the self-assembly process was driven by the interaction of hydrophobic patches on the surfaces of individual GFP molecules. In addition, Balazs’s simulated fibers closely corresponded with what Kowalewski observed using AFM imaging.

“Our protein-polymer system gives us an atomically precise, very well-defined nanoscale building object onto which we can attach different handles in very precisely defined positions. It can be used in a way that wasn’t ever intended by biology,” said Kowalewski.

© Carnegie Mellon News 

0 Comments

Login

Your Comments (Up to 2000 characters)
Please respect our community and the integrity of its participants. WOC reserves the right to moderate and approve your comment.

Related News


Lubrizol acquires medical device manufacturer, Vesta

CLEVELAND, US: The Lubrizol Corporation has acquired Vesta, a leading contract manufacturer for the global medical device industry. This acquisition b ...

Read more
American Securities acquires Emerald Performance Materials

OHIO, US: Emerald Performance Materials, LLC, has completed the sale of the company to an affiliate of private equity firm American Securities LLC. Te ...

Read more
New material to detect contaminated meat before reaching consumers

EDMONTON, CANADA: Meat getting poisoned with Salmonella or E. coli is the common worries of the meat eaters. Researchers at the University of Alberta ...

Read more
Arkema, Enterprise sign propylene supply agreement

COLOMBES, FRANCE: Arkema has reached an agreement for the supply of propylene with Enterprise Products Partners LP, a leading United States midstream ...

Read more
Nippon Shokubai cancels SAP project in China

TOKYO, JAPAN: Nippon Shokubai announced the cancellation of investment for superabsorbent polymers (SAP) at Nisshoku Chemical Industry, Zhangjiagang C ...

Read more
Environmentally friendly way to make coloured plastics

WASHINGTON DC, US: Long before humans figured out how to create colours, nature had already perfected the process – for example stunning, bright ...

Read more